Engineering antigen‐specific NK cell lines against the melanoma‐associated antigen tyrosinase via TCR gene transfer
نویسندگان
چکیده
منابع مشابه
SUSCEPTIBILITY OF HUMAN WM MELANOMA CELL LINES TO NK AND LAK CYTOTOXICITY AND THEIR RELEVANCE TO THE LEVEL OF MHC CLASS I AND ICAM-l ANTIGEN EXPRESSION
The effect of natural killer (NK) cells and lymphokine activated killer ( LAK) cells was studied on a group of human melanoma cell lines. Peripheral blood from healthy volunteers was utilized as a fresh source of natural killer cells and rhI L-2 for producing LAK cells. The cytotoxicity of effector cells was quantified using a 4 hour SI determining the density of antigen expression on tumor...
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NK cells are promising effectors for tumor adoptive immunotherapy, particularly when considering the targeting of MHC class I low or negative tumors. Yet, NK cells cannot respond to many tumors, which is particularly the case for nonhematopoietic tumors such as carcinomas or melanoma even when these cells lose MHC class I surface expression. Therefore, we targeted primary human NK cells by gene...
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EBV is associated with a broad range of malignancies. Adoptive immunotherapy of these tumors with EBV-specific CTL proved useful. We generated a panel of primary human T cells specific to various EBV antigens (i.e. Epstein-Barr nuclear antigen 3A, 3B and BamHI-M leftward reading frame) via transfer of modified TCR genes that are either coupled to CD3zeta or Fc(epsilon)RIgamma. TCR-transduced T ...
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EBV is associated with a broad range of malignancies. Adoptive immunotherapy of these tumors with EBV-specific CTL proved useful. We generated a panel of primary human T cells specific to various EBV antigens (i.e. Epstein–Barr nuclear antigen 3A, 3B and BamHI-M leftward reading frame) via transfer of modified TCR genes that are either coupled to CD3f or Fc(e)RIc. TCR-transduced T cells from 20...
متن کاملGeneration of diffuse large B cell lymphoma-associated antigen-specific Vα6/Vβ13+T cells by TCR gene transfer
BACKGROUND Our previous study had amplified antigen-specific full-length TCR α and β genes of clonally expanded T cells in the peripheral blood (PB) of patients with diffuse large B-cell lymphoma (DLBCL). The transfer of T cell receptor (TCR) genes endows T cells with new antigen specificity. Therefore, the aim of this study is to generate diffuse large B cell lymphoma (DLBCL)-specific T cells ...
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ژورنال
عنوان ژورنال: European Journal of Immunology
سال: 2019
ISSN: 0014-2980,1521-4141
DOI: 10.1002/eji.201948140